Scientist Profile

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Teresita Espino

Biochemist, Outstanding Microbiologist (2004)

Expertise: Biochemistry, Food Science, Biotechnology

Dr. Teresita M. Espino is recognized for her important contributions in the field of Biochemist (Director 2000-2006) drew upon her personal experience in commercializing Virgin Coconut Oil (VCO) products when she addressed the Institute’s current workforce. She said quality of products must continue to be improved. As an example, she narrated that she has improved on the shelf life of VCO by innovating on accepted procedures. Dr. Espino said that through the stages of product development: optimization, bench scale and large scale, it must be remembered that quality should be able to compete in the market. She talked about certainty in data, the need to conduct several trials and the need to maintain equipment. She further said that the institute should continue technologies with potential and put staff where they will be maximized.

Affiliation

Proprietress/Retired Research Professor, Mark Enterprises, Pilot Plant, Science and Technology Park, UPLB, Laguna

Association

    • Gamma Sigma Delta, Life Member
    • Fund Raising, Co-Chairman
    • American Dairy Science Association, Member
    • Institute of Food Technologies, Member
    • American Chemical Society, Member
    • Kapisanan ng mga Kemiko ng Pilipinas, Life Member
    • Kapisanan ng mga Kemiko ng Pilipinas, Board Member
    • Kapisanan ng mga Kemiko ng Pilipinas, Treasurer
    • Society for the Advancement of Research(SAR), Life Member
    • Society for the Advancement of Research (SAR), Auditor
    • Society for the Advancement of Research (SAR), Board of Director
    • Society for the balik Awardees, Inc., Treasurer
    • Phil. Society for Microbiologist, Life Member
    • Philippine Association for the Advancement of Science, Member
    • National Research Council of the Philippines, Associate Member
    • Philippine Academy of Microbiology, Diplomate Secretary/ Treasurer
    • Biotechnology Association Of the Philippines (BCP), Member
    • University of the Philippines, Research, Extension and Professional Staff Association, Inc., Member, (Former Chairman)
    • Maquiling Ladies Club, Member

Employment

    • Department of Botany, U.P. College of Agriculture, Assistant to a Plant Physiologist (one year), 1963-1964
    • U.P. College of Agriculture, Instructor in Chemistry at the Department of Chemistry, 1964-1969
    • Department of Food Science, NCSU, Raleigh, North Carolina, Chromatographer, 1969-1972
    • Department of Biochemistry, Biochemist, 1977-1982
    • BIOTECH, Researcher and Professor, 1982-2007

Award

    • Balik Scientist Award, Los Baños Science Community, 1982
    • Most Outstanding Development Award for Agriculture, PCARRD-DOST, 1989 Jl 12
    • 2nd Place, Best Paper Award , Production of Pectinases from Aspergillus ficuum, 1989 N 20 -21
    • 1st Place, Best Paper Award , Monoclonal Antibody Based Diagnostic Kits for Rapid Indexing of Plant Diseases, 1990 My 14-15
    • 2nd Place,Southern Tagalog Achievement in Research (STAR), , 1990 Jl 7
    • Outstanding Researcher Award , 83rd Foundation Day, UPLB, College, Laguna , 1992 Mr 6
    • Likha Award , Technology Application and Promotion Institute (TAPI), DOST , 1994 Jl 9-17
    • Diplomate , Philippine Academy of Microbiology , 1996 My 23
    • Gawad Saka Award for Outstanding Agriculturist , Department of Agriculture, 1996 My 31
    • 2nd Place, Best Poster Paper , Philippine Council for Health and Research Development (PCHRD, 1996 Jl 15-18
    • Best Paper Award (Technology for Dissemination Category) , STARRDEC, 9th Annual Symposium , 1996 S 12
    • 3rd Place, Best Poster Paper Award, STARRDEC. 9th Annual Symposium , 1996 S 12
    • 2nd Place, Best Poster Paper Award, Bureau of Agricultural Research (BAR) , 1996 D 3-6
    • Outstanding Research in Industry and Energy , PCIERD at the 1st Regional and National S & T Fora and Competition on Industry and Energy Research, 1997 Ja 30
    • Outstanding Research in Industry and Energy , PCIERD 1st Regional and National S & T Fora and Competition on Industry and Energy Research , 1997 Mr 17
    • M.S. Swaminathan (Research Category) , Los Baños Science Community Convocation , 1998 Jl 16
    • Best Poster Paper- Third Place (Professional Category), Philippine Council for Health and Research Development (PCHRD) ., 1999 Jl 5-9
    • KKP-LB Distinguished Achievement Award , Kapisanang Kimika ng Pilipinas Sangay sa Los Baños , 2000 O 25
    • Golden Jubilee Gregorio Y. Zara Award for Applied Research , Philippine Association for the Advancement of Science (PHILAAS) , 2001S 13
    • 2001 Outstanding Scientific Paper Award, National Academy of Science and Technology (NAST) ., 2001 Jl 12
    • 1st Prize, LIKHA Award for Outstanding Creative Research in Food and Agriculture , Technology Applications and Promotion Institute, Department of Science and Technology (TAPI-DOST) , 2001 N 15-21
    • Award for Excellence in Biochemistry Research , Philippine Society of Biochemistry and Molecular Biology (PSBMB), 2001 D 3
    • DA Secretary's Award , Bureau of Agricultural Research, Department of Agriculture, 2002 N 28
    • 2002 International Publication Award , University of the Philippines, 2003 Mr 5
    • Fellow , Philippine Academy of Microbiology , 2003 My 15
    • First Place, National Research Paper Award in Agriculture and Natural Resources, , 2003 N 10
    • 2004 Outstanding Microbiologist , Philippine Society for Microbiology, Inc., 2004 Ap 28-30
    • Outstanding CA Extension Team award , UPLB College of Agriculture , 2005 Mr 4
    • 1st Prize, AFMA R & D Paper Award , 18th National Research Symposium, 2006 O 4-5
    • 2nd Prize AFMA R & D Paper Award , 18th National Research Symposium , 2006 O 4-5
    • Post Doctoral Fellow, Department of Food Science, 1972-1975
    • Post Doctoral Fellow, Department of Chemical Engineering, 1975-1976

Research

    • Amylase producing bacteria [Completed]
    • Isolation and comparison of cellulases from different sources (Study 5.4.6) [Completed]
    • Microbial production of food enzymes (Pectinases) [Completed]
    • Purification and characterization of cellulases-lignocellulose bioconversion [Completed]
    • Research materials substitution special Committee, use of reclaimed agar in microbiological media [Completed]
    • Serologically based diagnostic kits for rapid detection of rice tungro virus and other rice diseases [Completed]
    • Development of monoclonal antibody based techniques using enzyme linked immunosorbent assay (ELISA) and other methods for detection of food and feed toxins [Completed]
    • Liquid culture fermentation of Aspergillus ficuum for enhanced pectinase production (Study 3. Application of the produced pectinase on essential oil extraction, fruit juice clarification and wine making) [Completed]
    • Screening, optimizatrion of conditions for production, purifiction and application of xylanase [Completed]
    • Enzymatic degumming of selected fiber crops [Completed]
    • Monoclonal antibodies for diagnosis of selected plant viral, fungal and bacterial diseases [Completed]
    • Development of rapid screening method for detection of leaf mottling in citrus using monoclonal antibodies [Completed]
    • Development of rapid indexing method for detection of leaf mottling and tristeza virus in citrus [Completed]
    • Bioprocessing of ramie [Completed]
    • Monoclonal antibodies for rapid indexing of virus diseases in Musa spp. [Completed]
    • Microbial production of food enzymes (Lipases) [Completed]
    • Microbial screening and process optimization for ligninase production [Completed]
    • Monoclonal antibodies for rapid screening of aflatoxin B1 and M1 in food and feed products [Completed]
    • Serologically based diagnostic kits for detection of plant diseases (Monoclonal antibodies for diagnosis of diseases of economically important crops) [Completed]
    • Production of diagnostic kits for indexing viral diseases of identified fruits [Completed]
    • Monoclonal antibody based detection technique for monitoring red tide toxin causal organism/red tide toxins (Phases I and II) [Completed]
    • Pilot production and utilization of pectic enzymes in food (banana and pineapple) applications [Completed]
    • Development of rapid indexing technique for virus diseases in banana through monoclonal antibody technology [Completed]
    • Large scale application of lipases for industrial applications [Completed]
    • Large scale production of microbial proteases for food and other industrial applications (Phase I) [Completed]
    • Establishment of laboratories for monitoring red tide incidence in the Philippines using serologically based diagnostic kit against red tide toxins [Completed]
    • Studies on the application of BIOTECH produced lipases on enzyme catalyzed synthesis of flavor esters and coconut oil based products [Completed]
    • Development of rapid diagnostic kits for aflatoxin B1, zearalenone and fumonisin B1 (Phase II) [Completed]
    • Development of diagnostic kits for detection of banana bract mosaic virus and banana mosaic virus through monoclonal antibody technology [Completed]
    • Production and validation of diagnostic kits for indexing banana bunchy top virus and training [Completed]
    • Monoclonal antibody based detection technique for monitoring red tide toxin causal organism(s)/red tide toxins (Phase III) [Completed]
    • High value products from starches of root crops by enzyme Biotechnology [Completed]
    • Application of enzyme Biotechnology for essential oil production from different plant sources [Completed]
    • Field trial of detection kits for plant viruses and vaccines for livestock and poultry [Completed]
    • Studies on the application of cellulase for feed and other food industries (Phase 1) [Completed]
    • Application of BIOTECH produced proteases for food, feed and other industries (Phase 1) [Completed]
    • Development of locally produced detection methods for genetically modified corn and soybean [Completed]
    • Production of monoclonal anibodies for indexing virus diseases and training/support system [Completed]
    • Development of monoclonal antibody based technology for the detection of abaca bunchy top virus (ABTV), Abaca mosaic virus (AMV) and Abaca bract mosaic virus (ABMV) [Completed]
    • National Immunological Testing Laboratory [Completed]
    • Application of BIOTECH produced microbial proteases for food, feeds and other industries (Phase II) [Completed]
    • Utilization of BIOTECH cellulase as feed additive for poultry and swine diets and other application studies (Phase II) [Completed]
    • Income Generating Projects: Virgin coconut oil, Virgin coconut oil soap, Essential oils and industrial enzymes [On going]
    • Mass production of disease free abaca and banana plantlets [On going]
    • Mycotoxin determination by ELISA Enzyme-based assays [Completed ]

Papers Presented

    • Comparative Studies on the Ligninolytic and Cellulolytic Enzymes of Phanerochaete Chrysosporium and PICOP WRA Isolate , 19th PSM Annual Convention, Hotel Intercontinental, Makati, Metro Manila
    • Biotechnological Technique of Detecting Disease Causing Organisms in Plant Food and Feed Products , Training Center, DSAC, Indang, Cavite
    • Optimization of conditions for production of lipolytic enzymes from Rhizopus delemar under solid substrate fermentation. , 22nd PSM Annual Convention, University of San Agustin, Iloilo City
    • Seed Transmissibility of Papaya Ringspot Virus, 6th FCSSP Annual Conference Aristocrat Hotel, Naga City
    • Monoclonal Antibodies as Tools for Prevention and Eradication of Bunchy Top Virus in Banana , 6th FCSSP Annual Conference, Aristocrat Hotel, Naga City
    • Detection of Ringspot Virus in Papaya Seeds , PCCP PPS Annual Convention, Bacolod City
    • Purification of Citrus Tristeza Virus and Production of Antiserum and Initial Hybridomas for Diagnosis of Citrus Tristeza Virus Using ELISA Techniques , FSCCP Annual Conference, La Trinidad, Benguet
    • High pressure Liquid Chromatography, KKP LB Annual Convention, FPRDI, College, Laguna
    • Effect of UHT on the L lactalbumin and  lactoglobulin of Milk , KKP Annual Convention, Ding Hao Restaurant, Calamba, Laguna
    • Characterization of Pectin Transeliminase and Polygalacturonase from Locally Isolated Aspergillus ficuum , 17th PSM Annual Convention Hotel Intercontinental, Makati, Metro Manila
    • Purification, Characterization and Use of Monoclonal Antibodies for Early Detection of Bunchy Top , Symposium sponsored by the Phil. Phytopathological Society, Davao City
    • Production of Pectinases from Aspergillus ficuum, 28th Philippine Association of Food Technologists, Inc., Annual Convention, PICC, Manila
    • Diplocyclos Palmatus L: A New Weed Host of Papaya Ringspot Virus , PCCP PPS Annual Conference, Bacolod City
    • Indexing Papayas for Plant Viruses, Bureau of Plant Industry, BPI San Andres, Manila
    • Bioprocessing of Ramie, Los Baños, Laguna
    • Isolation and Screening of Lipase Producing Microorganisms, 21st PSM Annual Convention, United Laboratories Compound, Mandaluyong, Rizal
    • Characterization of the Causal Organism of Anthurium Blight, 21st PSM Annual Convention, United Laboratories Compound, Mandaluyong, Rizal
    • Isolation and Screening of Protease Producing Bacteria, 21st PSM Annual Convention, United Laboratories
    • Isolation, Screening and Characterization of Pectinase Bacteria and their Application on Degumming of Ramie (Boehmeria nivea Gaud) , FSCCP Annual Conference, La Trinidad, Benguet
    • Evaluation of White Rot Fungal Isolate and Mutants for Ligninase Production, 22nd PSM Annual Convention, University of San Agustin, Iloilo City
    • Isolation, Purification and Characterization of Neutral Protease from Bacillus subtilis (Ehrenberg) Cohn Biotech 1333) , 22nd PSM Annual Convention, University of San Agustin, Iloilo City
    • Optimization of Methyl Laurate Preparation Using Immobilized Lipase from Rhizopus delemar , 23rd PSM Annual Convention, Century Park Sheraton, Manila
    • Isolation, Purification and Characterization of Alkaline Protease from Bacillus subtilis NRRL-B3749 (BIOTECH 1333) , 23rd PSM Annual Convention, Century Park Sheraton, Manila
    • Production of short chain flavor esters from locally produced microbial lipases , 23rd PSM Annual Convention, Century Park Sheraton, Manila
    • Optimization of Conditions for Maximum Lipase Production in Laboratory Scale Using Solid Substrate Fermentation of Rhizopus arrhizus , 23rd PSM Annual Convention, Century Park Sheraton, Manila
    • Optimization of Conditions for Laboratory and Bench Scale Acid Protease Production by Solid Substrate Fermentation Using Monascus anka var rubellus 102 BIOTECH 3064 , 23rd PSM Annual Convention, Century Park Sheraton, Manila
    • Purification and Partial Characterization of Lipases from Rhizopus delemar , 23rd PSM Annual Convention, Century Park Sheraton, Manila
    • Purification of Acid Protease from Monascus anka var Rubellus 102 BIOTECH 3564 and Economic Analysis of its Production , 24th PSM Annual Convention, Xavier University, Cagayan de Oro City
    • Purification of the Red Tide Toxin from Frateuria sp. Isolated from Pyrodinium bahamanse var Compressum , 24th PSM Annual Convention, Xavier University, Cagayan de Oro City
    • Partial Purification and Characterization of Pectinases of Aspergillus ficuum Isolated from Pomelo , 24th PSM Annual Convention, Xavier University, Cagayan de Oro City
    • Strain Improvement of Rhizopus delemar Using Ultraviolet Light and Nitrosoguanidine Treatments for Increased Lipolytic Enzyme Production , 24th PSM Annual Convention, Xavier University, Cagayan de Oro City
    • Ligninase Production by Polyurethane Immobilized Phanerochaete Chrysosporium Mutant for Bleaching of Abaca Kraft Pulp , 24th PSM Annual Convention, Xavier University, Cagayan de Oro City
    • The Importance of Monoclonal Antibody Technology in Relation to the General Agreement on Tariff and Trade (GATT) , 1st PSM Annual Convention, Visayas Chapter, U.P. in the Visayas Campus, Miag-ao, Iloilo
    • Enzyme Biotechnology Towards Philippines 2000, 12th Philippine Federation of the Chemical Societies Annual Anniversary, Sarabia Hotel, Iloilo City
    • Application of Pectinase in the Production of Clarified Juice, 26th PSM Annual Convention, Caliraya Recreation Center, Lewin, Lumban, Laguna
    • Isolation, Transmission and Purification of Potato Virus X and Y, 26th PSM Annual Convention, Caliraya Recreation Center, Lewin, Lumban, Laguna
    • Determination of Distribution of Papaya Ringspot Virus (PRSV)on Different Plant Parts , 26th PSM Annual Convention, Caliraya Recreation Center, Lewin, Lumban, Laguna
    • Transmission of Banana Bract Mosaic Virus (BBMV) and Banana Mosaic Virus (BMV) , 26th PSM Annual Convention, Caliraya Recreation Center, Lewin, Lumban, Laguna
    • Monoclonal Antibody Technology, 25th KKP-LB Annual Convention (Silver Anniversary)SEARCA Auditorium, UPLB, College, Laguna
    • Potential Application of BIOTECH Produced Pectinases for the Local Food Industry , 36th Annual PAFT Convention, Manila Galleria Suites, Robinson EDSA, Mandaluyong City
    • Studies on the application of BIOTECH produced lipases on enzyme catalyzed synthesis of flavor esters and coconut oil based products , 10th year Anniversary, PCASTRD, Midtown Hotel, Manila
    • Application of Pectinases in the Extraction of Essential Oils from Ilang-ilang flowers (Cananga odorata) , 27th PSM Annual Convention, The Manila Hotel, Rizal Park, Manila
    • Immobilization of pectinase for banana juice production, 27th PSM Annual Convention, The Manila Hotel, Rizal Park, Manila
    • Application of Enzyme Biotechnology in Agriculture, 25th Anniversary and Annual Convention Philippine Society of Biotechnology and Molecular Biology, Marine Science Institute, UP Diliman, Quezon City
    • Lipase Catalyzed Synthesis of an Antimicrobial Agent from Coconut Oil , 28th PSM Annual Convention, Asturias Hotel, Puerto Princesa City, Palawan City
    • Isolation, Screening and Characterization of High Yielding Alpha-amylase Producing Bacteria , 28th PSM Annual Convention, Asturias Hotel, Puerto Princesa City, Palawan
    • Optimization of Conditions for Maximum α-amylase Production by Liquid Substrate Fermentation with Bacillus amyloliqufaciens ATCC 23350 as Organism , 28th PSM Annual Convention, Asturias Hotel, Puerto Princesa, City, Palawan
    • Isolation, Screening and Characterization of glucoamylase producing microorganisms , 28th Annual Convention of the Philippine Society for Microbiology, Inc.
    • Acid Protease from Monascus sp. BIOTECH 3064 and Other Microbial Enzymes as Feed Additive in Broiler Diets , 28th Annual Convention of the Philippine Society for Microbiology, Inc.
    • Application of Fungal Pectinase on the Extraction of Essential Oils from Pathouli (Pogostemon cablin) , 28th Annual Convention of the Philippine Society for Microbiology, Inc.
    • Biotechnology and Natural Products, 5th Annual Convention of the Natural Products Society of the Philippines, EO Tan Hall, PCARRD, Los Baños, Laguna
    • Production of High Value Products from Cassava (Manihot esculenta) and Sweet Potato (Ipomea batatas) Starches by Enzymatic Hydrolysis , 4th Asia-Pacific Biotechnology Congress and 30th Annual Philippine Society for Microbiology, Inc. Convention
    • Industrial Applications of Biotechnology Research in the Philippines , 4th Asia Pacific Biotechnology Congress and 30th Annual Philippine Society for Microbiology, Inc. Convention
    • The Promise of Biotechnology: Elevating the Industry and Protecting the Environment, Two-day Information Seminar on Modern Biotechnology Auditorium of the Thomas Aquinas Research Center, UST
    • Potential Use of BIOTECH Produced -Monoglyceride as Protectant of Tomato, Cabbage and Chinese Cabbage gainst Soft Rot Disease , 31st Philippine Society for Microbiology, Inc. Annual Convention
    • Efficacy of Biotech-Derived Kit for Aflatoxin B1 Detection In Food and Feed Products , Proceedings of the 31st Philippine Society for Microbiology, Inc. Annual Convention
    • Utilization of Pectinase from Aspergillus sp. in Extraction of Banana Juice , 31st Philippine Sociey for Microbiology, Inc. Annual Convention
    • Culture Conditions for the Production of -Amylase from Rice Bran , 31st Philippine Society for Microbiology, Inc. Annual Convention
    • Production of Maltodextrin and Glucose from Sweet Potato Starch by Enzyme Hydrolysis using Iso-1 Bacterial -Amylase and a Fungal Glucoamylase , 31st Philippine Society for Microbiology, Inc. Annual Convention
    • Supplementation of Corn-Soybeans Based Broiler Diets with Different Levels of Acid Protease , Philippine Society of Animal Science 39th National and 20th Visayas Chapter Scientific Seminar and Annual Convention Marriott Hotel, Cebu City
    • Biotechnology in the Forefront of Agriculture and Fisheries Modernization Plan (AFMA) , Kapisanang Kimika ng Pilipinas (KKP) 31st Annual Convention ERDB Auditorium, UPLB Forestry campus, College, Laguna
    • Industrial Applications of Biotechnology Research, Philippine Society for Biochemistry and Molecular Biology (PSBMB), SEARCA Auditorium, College, Laguna
    • Utilization of Alpha-Amylase from Aspergillus sp. and Neutral Protease from Bacillus sp. in Pandesal Making , 32nd Philippine Society for Microbiology, Inc. Annual Convention
    • Enzymatic Extraction of Essential Oils from Bungulan (Musa sapeintum var suaveotens) and Cavendish (Musa cavendishii) Banana Peels Using Pectinase , 32nd Philippine Society for Microbiology, Inc. Annual Convention
    • Monoclonal Antibody Based Enzyme Linked Immunosorbent Assay (ELISA) Technique for Detection of Economically Important Plant Pathogen in the Philippines , Thomas Aquinas Research Center, UST
    • Diagnostic Kit for Red Tide Toxin , Seminar/Workshop sponsored by PSM Visayas Chapter Talamban, Cebu City
    • Efficacy of BIOTECH Diagnostic Kit for Aflatoxin B1 Detection in Food and Feed Products , PCARRD-DOST National Symposium and Awarding Ceremonies, Centennial Hall, Manila Hotel
    • Development of Rapid Diagnostic Kit for Food and Feed Toxins, Philippine Association of Food Technologists, Manila Galleria
    • Local Production of U.S.P. Grade Dextrose from Corn Starch and Cassava Starch for Intravenous (I.V.) Fluids Via Enzymatic Hydrolysis using -amylase and Glucoamylase , 33rd Philippine Society for Microbiology, Inc. Annual Convention
    • Improved Starch Extraction from Different Root Crops., 34th Philippine Society for Microbiology, Inc. Annual Convention.
    • Virgin Coconut Oil (Technical Aspect), 44th PAFT Annual Convention, Crowne Plaza, Galleria Manila, ADB Avenue, Quezon City
    • Biotechnology Process for Virgin Coconut Oil Production, Tagbilaran, Bohol
    • Partial Purification and Characterization of Acid Protease, Tagbilaran, Bohol
    • Purification and Characterization of Xylanase , Tagbilaran, Bohol
    • Development and Optimization of Solid Substrate Cultivation of Pectic Enzymes Using Local Isolates , 7th World Congress of Food Science and Technology, Singapore
    • Isolation, Screening and Characterization of Pectinase Producing Fungi , Asia-Pacific Biotechnology Congress, PSM Manila Garden Hotel, Manila, Philippines
    • Purification of Pectinases and Optimization of Conditions for Production of Aspergillus ficuum , Asia-Pacific Biotechnology Congress organized by PSM Manila Garden Hotel, Manila, Philippines
    • Microbial Production of Pectinases from Aspergillus ficuum, Osaka, Japan
    • Development of Hybridoma Based Diagnostic Kits for Plant Virus Diseases of Some Fruit Crops , Philippine International Convention Center, Manila
    • Optimization of Process Conditions on Fungi Production of Pectic Enzymes in a Solid State Fermentation , 8th International Conference on Global Impacts of Applied Microbiology and International Conference on Applied Biotechnology and Biology, Hongkong
    • Isolation and Characterization of the Citrus Greening or Leaf Mottling Organism for the Production of Monoclonal Antibodies , Biotechnology Chiang Mai University, Chiang Mai, Thailand
    • Rapid Indexing of Banana Bract Mosaic Virus Using Serological Techniques , Merida, Venezuela
    • Purification and Isotyping of Monoclonal Antibodies Against Some Important Fruit Diseases , 2nd Asean Science and Technology Week, Westin Philippine Plaza, Manila, Philippines
    • Monoclonal Antibodies as Tools for Diagnosis of Selected Fruit Crop, 2nd SSM International Congress for Microbiology Pan Pacific Hotel, Singapore
    • Purification and Properties of Polygalacturonase from Aspergillus ficuum, NRCT, NUS, DOST JSPS Joint Seminar of Biotechnology, Thailand and Singapore
    • Mutation, Induction and Evaluation for Resistance to Leaf Mottling in Citrus , Final FAO/IAEA Research Coordination Meeting, Grunback, Germany
    • Detection of Cymbidium Mosaic Virus and Tobacco Mosaic Virus Orchid Strain by Enzyme Linked Immunosorbent Assay , NRCT, NUS, DOST JSPS Joint Seminar on Biotechnology, SIAM Hotel, Bangkok, Thailand
    • Optimization of conditions for protease production using Bacillus subtilis (Ehrenberg) Cohn BIOTECH 1333 , NRCT, NUS, DOST JSPS Joint Seminar on Biotechnology,Siam Hotel, Bangkok, Thailand
    • Application of monoclonal antibodies in identification of Plant and food pathogens , DOST Compound, Bicutan, Taguig, Metro Manila
    • Immunological techniques for detection of pathogens of some economically important crops in the Philippines , International Symposium on the 20th Anniversary of International Post Graduate University Course in Microbiology, Osaka, Japan
    • Prospects for Commercialization of Antibodies/Diagnostic Kits for Rapid Detection of Pathogens of Economically Important Crops in the Philippines , Heritage Hotel, Roxas Boulevard corner EDSA, Metro Manila, Philippines
    • A Rapid, Sensitive and Economical Method of Aflatoxin B1 Detection in Food and Feed Products , 3rd Asia Pacific Biotechnology Congress and 25th PSM Annual Convention, The Peninsula Manila, Philippines
    • Rhizopus delemar Lipase Catalyzed Interesterification of Coconut Oil and Corn Oil , 3rd Asia Pacific Biotechnology Congress and 25th PSM annual Convention, The Peninsula Manila, Philippines
    • Improved Starch Extraction from Different Root Crops Using Cellulase from Trichoderma sp. and Pectinase from Aspergillus sp., 34th Philippine Society for Microbiology, Inc. Annual Convention
    • Screening and Optimization of Conditions for Xylanase Production from Bacterial Isolates., 34th Philippine Society for Microbiology, Inc. Annual Convention
    • Enzymatic Extraction of Essential Oil from Sampaguita Flowers (Jasminum sambae (L.) Alton) Using Pectinase from Aspergillus sp. , 33rd Philippine Society for Microbiology, Inc. Annual Convention
    • Enzymatic Extraction of Essential Oils from Calamansi (Citrus microcarpa Bunge) Rind Using Pectinase from Aspergillus sp., 34th Philippine Society for Microbiology, Inc. Annual Convention
    • Culture Conditions for the Production of -Amylase from Rice Bran by Aspergillus sp., Culture Conditions for the Production of -Amylase from Rice Bran by Aspergillus sp
    • Potential Use of Biotech Produced -Monoglyceride as Protectant of Tomato, Cabbage and Chinese Cabbage against Soft Rot Disease, 31st Philippine Society for Microbiology, Inc. Annual Convention
    • Efficacy of Biotech-Derived Kit for Aflatoxin B1 Detection in Food and Feed Products, 31st Philippine Society for Microbiology, Inc. Annual Convention
    • Utilization of Pectinase from Aspergillus sp. in Extraction of Banana Juice, 31st Philippine Society for Microbiology, Inc. Annual Convention
    • Production of Maltodextrin and Glucose from Sweet Potato Starch by Enzyme Hydrolysis using Iso-1 Bacterial -Amylase and a Fungal Glucoamylase, 31st Philippine Society for Microbiology, Inc. Annual Convention
    • Production of high value products from cassava (Manihot esculenta) and sweet potato (Ipomea batatas) starches by enzymatic hydrolysis, 4th Asia-Pacific Biotechnology Congress and 30th Annual Philippine Society for Microbiology, Inc. Convention
    • Application of Pectinases in the Extraction of Essential Oils from Ilang-ilang (Cananga odorata) Flowers, 27th Annual Convention of the Philippine Society for Microbiology
    • Prospects for Commercialization of Antibodies/Diagnostic Kits for Rapid Detection of Pathogens of Economically Important Crops in the Philippines , International Symposium on Development of Small and Medium Enterprises for Biotechnology Commercialization in Developing Countries
    • Immunological techniques for detection of pathogens of some economically important crops in the Philippines, International Cooperation and Education in Applied Microbiology, UNESCO
    • Isolation, transmission and purification of potato viruses X and Y, 26th Annual Convention of the Philippine Society for Microbiology
    • Indexing Papayas for Plant Viruses, National Symposium Workshop on Ringspot and other Diseases of Papaya in the Philippines
    • Rapid Indexing of Banana Bract Mosaic Virus(BBMV) Using Serological Techniques, ACORBAT 89 IX Meeting
    • Industrial Applications of Biotechnology Research in the Philippines, 4th Asia Pacific Biotechnology Congress and 30th Annual Philippine Society for Microbiology, Inc. Convention
    • Immobilization of pectinase for banana juice production, 27th Annual Convention of the Philippine Society for Microbiology

Education

    • University of the Philippines-Diliman , Diliman, Quezon City, Philippines , B.S. Chemistry, 1963
    • University of the Philippines College of Agriculture , Los Baños, Laguna, Philipines, M.S. Agricultural Engineering, 1969
    • North Carolina State University (NCSU) , Rayleigh, North Carolina, USA, Ph.D. in Food Science, 1972

Publication

    • Tove, S.B., Hunter, S.W., Butz, T.M. , (1980) Stimulation of triacylglycerol synthesis by intracellular serum albumin
    • Archives of Biochemistry and Biophysics Volume 204
    • Hansen, Melo, T.S. , (1977) Effects of ultra high temperature direct steam injection upon constituents of skim milk
    • Journal Dairy Science Volume 60
    • Melo, T.S., Hansen, A.P., (1978) Effects of ultra high temperature direct steam injection on lactalbumin and lactoglobulin
    • Journal of Dairy Science Volume 61
    • Melo, T.S., Blumer, T.N., Swaisgood, H.E., Monroe, R.J., (1974) Catheptic enzyme activity in aged country style hams as influenced by precuring treatments
    • Journal of Food Science Volume 39
    • Espino, T.M., Exconde, S.B., San Luis, M.M., Ramirez, J.A. III. , (1986) Purification of citrus tristeza virus and production of antiserum and initial hybridomas for diagnosis of citrus tristeza virus using ELISA technique.
    • Philippine Journal of Crop Science Volume 11:
    • Espino, T.M., Exconde, S.B., Zipagan, F.B., Maroon, M.C.M., Espino, R.R.C. , (1989) Production of monoclonal antibodies for diagnosis of banana bunchy top virus
    • Philippine Agriculturist Volume 72 , No. 4
    • Espino, T.M., Exconde, S.B., Zipagan, F.B., Espino, R.R.C. , (1989) Production of monoclonal antibodies against abaca mosaic virus
    • Philippine Ariculturist Volume 72, No. 4
    • Espino, T.M., Exconde, S.B., Zipagan, F.B., Espino, R.R.C. , (1990) Banana bract mosaic, a new disease of banana II. Isolation and purification for monoclonal antibody production
    • Philippine Agriculturist Volume 73, No. 1
    • Tapay, L.M., Mercado, M.A., Mercado, S.M., Espino, T.M., (1990) Isolation and identification of fungi for pectinase production
    • Philippine Journal of Biotechnology Volume 1, No. 1
    • Espino, T.M., Mercado, S.M., Alcala, M.A., Tapay, L.M., (1990) Purification and characterization of pectin transeliminase and polygalacturonase from locally isolated Aspergillus ficuum
    • Philippine Journal of Biotechnology Volume 1 , No. 1
    • Ramirez, M.J.C., Audije, M.B.R., Tammarato, P., Espino, T.M., del Rosario, E.J., (1993) Evaluation of white-rot fungal isolates and mutants for ligninase production
    • Philippine Journal of Biotechnology Volume 4 , No. 1
    • Centeno, R.M., Espino, T.M., Mercado, M.A., (1996) Purification and partial characterization of alkaline protease from Bacillus subtilis NRRL B-3749 (BIOTECH 1679)
    • Philippine Journal of Biotechnology Volume 7, No. 1
    • Espino, T.M., Sanchez, P.C., Mercado, M.A., Escaño, C.S., Descallar, C.V. , (1997) Industrial applications of locally produced pectinases
    • Philippine Technology Journal Volume 22, No. 1
    • Espino, T.M., Sanchez, P.C., Mercado, M.A., Escaño, C.S. , (1998) Potential applications of BIOTECH produced pectinases for the local food industry
    • Philippine Journal of Food Science and Technology Volume 20, No. 1
    • Exconde, S.B., Raymundo, A.K., Espino, T.M. , (2000) Development of a nucleic acid-based monoclonal antibody technique for the detection of the bacterial wilt organism in banana in the Philippines
    • Philippine Agricultural Scientist Volume 83, No. 2
    • Bajo, L.M., Castillo, E.M., Espino, T.M. , (2000) Immobilization of glucoamylase on silica gel for the hydrolysis of cassava starch
    • Philippine Agricultural Scientist Volume 83, No. 2
    • Espino, T.M., Arevalo, R.E., Sapin, A.B., Tambalo, F.Z., Rico, C.M. , (2002 S) Enzymatic extraction of essential oil from the leaves of patchouli (Pogostemon cablin Blanco,Benth
    • Philippine Agricultural Scientist Volume 85, No. 3
    • San Luis, M.M. Espino, T.M. Kinoshita, S. Taguchi, H., (1985) Purification of cellulolytic enzymes from Sporotrichum cellulophilum.
    • Annual Reports of IC Biotech Volume 8
    • Espino, T.M. Pham, C.B. Tapay, L.M. Maldo, S.M. Alcala, M.A. Alba, D.M. Molina, N.T. Lapitan, H.A. , (1986) Microbial production of pectinases
    • Microbial Utilization of Renewable Resources Volume 5
    • Espino, T.M., Exconde, S.B. Ito, K. Iisuka, M. Minamiura, N. , (1986) Production of monoclonal antibodies against citrus tristeza and papaya ringspot virus
    • Annual Reports of Institute of Chemistry (IC) Biotech Volume 9
    • Espino, T.M., Exconde, S.B., Ito, K., Iisuka, M., Minamiura, N. , (1987) Production of antiserum and initial hybridomas against papaya ringspot virus
    • Transactions of National Academy Science and Technology (Philippines) Volume 9
    • Espino, T.M., Minamiura, N., (1988) Preparation of monoclonal antibody against virus and papaya ringspot virus
    • Annual Reports of IC Biotechnology
    • Espino, T.M., Exconde, S.B., Mercado, S.M., Bite, M.F.P. , (1989) Papaya ringspot virus: Diagnostic kit to determine virus distribution in papaya plants
    • The PCARRD Monitor Volume 17, No. 11
    • Espino, T.M., Bite, M.F.P., Exconde, S.B. , (1989) Isolation and characterization of citrus greening or leaf mottling organisms
    • Microbial Utilization of Renewable Resources Volume 6
    • Espino, T.M., Minamiura, N. , (1989) Determination of molecular weight of monoclonal antibodies against various viruses
    • Annual Reports of IC Biotech Volume 12
    • Espino, T.M., Minamiura, N., Iisuka, M., Ito, K. , (1989) Purification of pectin degrading enzymes of Aspergillus ficuum
    • Annual Reports of IC Biotechnolgy Volume 12
    • Espino, T.M., Exconde, S.B., Maldo, S.M., Bite, M.F.P., Zipagan, F.B., (1990) Purification and istotyping of monoclonal antibodies against some important fruit diseases
    • 2nd Asean Science and Technology Week Proceedings Volume 2
    • Espino, T.M., Exconde, S.B. , (1993) Detection of cymbidium mosaic virus and tobacco mosaic virus orchid strain by enzyme linked immunosorbent assay
    • Microbial Utilization of Renewable Resources Volume 8
    • Espino, T.M., Mercado, M.A., Mercado, S.M, (1993) Optimization of conditions for protease production using Bacillus subtilis (Ehrenberg) Cohn BIOTECH 1333
    • Microbial Utilization of Renewable Resources Volume 8
    • Espino, R.R.C., Estrella, J.B., Mitra, V.T., Espino, T.M., (1993) Mutation, induction and evaluation for resistance to leaf mottling in citrus
    • Joint FAO/IAEA
    • Espino, T.M., Tambalo, R.D., Elegado, F.B. , (1996) Microbial production of a-amylase for food and other industrial applications
    • Annual Reports of IC Biotechnology Volume 19
    • Espino, T.M., Tambalo, R.D. , (1997) Isolation, screening and characterization of high yielding -amylase producing bacteria
    • Annual Reports of IC Biotechnology Volume 20
    • Espino, T.M., Tambalo, R.D. , (1998) Optimization of conditions for maximum -amylase production by liquid culture fermentation with Bacillus amyloliquefaciens ATCC 23350 as organism
    • Annual Reports of IC Biotechnology Volume 21
    • Espino, T.M., Jacob, J.D.C., Tambalo, F.Z., Tambalo, R.D. , (2001) Production of maltodextrin and glucose from cassava starch using D-Amylase and Gluco-Amylase from Philippine isolates
    • Biotechnology for Sustainable Utilization of Biological Resources in the Tropics Volume 15
    • Sapin, A.B., Espino, T.M., Ito, K., Iizuka, M. , (2004 D) Partial purification and characterization of acid protease from Rhizopus sp.
    • Biotechnology for Sustainable Utilization of Biological Resources in the Tropics Volume 17
    • Hansen, A.P., Melo, T.S. , (1973 ) Effects of ultra high temperature direct steam injection upon constituents of skim milk
    • Journal of Dairy Science Volume 56
    • Melo, T.S., Hansen, A.P. , (1974) Effects of ultra high temperature direct steam injection on L - lactalbumin and  lactoglobulin
    • Journal of Dairy Science Volume 57
    • Melo, T. S., Hansen, A.P., Jones, V.A. , (1975 ) Effects of different temperatures and holding times on L lactalbumin and  lactoglobulin of skim milk
    • J. Dairy Sci. Volume 58
    • Melo, T.S., Hansen, A.P. , (1976 ) Effects of ultra high temperature steam injection on xanthine oxidase in skim milk
    • Journal of Dairy Science
    • Melo, T.S., Hansen, A.P. , (1976) Effects of ultra high temperature steam injection on the formation of milk deposit
    • J. Dairy Sci.
    • Espino, T.M., Bite, Ma. F.P., Exconde, S.B. , (1991) Characterization of the causal organism of anthurium blight
    • Philippine Jornal of Biotechnology Volume 2, No. 2
    • Espino, T.M., Tambalo, R.D., Patricio, V.V. , (1991) Isolation and screening of lipase producing bacteria
    • Philippine Journal of Biotechnology Volume 2, No. 2
    • Espino,T.M., Mercado, S.M., Mercado, M.A. , (1991) Isolation and screening of protease producing bacterial
    • Philippine Journal of Biotechnology Volume 2, No. 2
    • Oberes, A., Mercado, S.M., Mercado, M.A., Espino, T.M. , (1992) Isolation, purification and characterization of neutral protesase from Bacillus subtilis Ehrenberg (Cohn) Biotech 1333
    • Philippine Journal of Biotechnology Volume 3, No. 1
    • Ramirez, MJ.C., Audije, MB.R., Tammarate, T.M. Espino, T.M., del Rosario, E.J., (1992) Evaluation of white-rot fungal isolates and mutants for ligninase production
    • Philippine Journal of Biotechnology Volume 3, No. 1
    • Espino, T.M., Santos, M.C.T., Tambalo, R.D., Collado, B.R., (1993) Purification and partial characterization of lipases from Rhizopus delemar
    • Philippine Journal of Biotechnology Volume 4, No. 2
    • Espino, T.M., Baja, E.S., Tambalo, R.D. , (1993) Optimization of conditions for maximum lipase production in laboratory scale using solid substrate fermentation of Rhizopus arrhizus
    • Philippine Journal of Biotechnology Volume 4, No. 2
    • Duldulao , A.M.D., Castillo, E.M., Espino, T.M., Tambalo, R.D., Padolina, W.G. , (1993) Production of short chain flavor esters from locally produced microbial lipases
    • Philippine Journal of Biotechnology Volume 4, No. 2
    • Centeno, R.M., Espino, T.M., Salamat, M.B. , (1993) Isolation, purification and characterization of alkaline protease from Bacillus subtilis
    • Philippine Journal of Biotechnology Volume 4, No. 2
    • Macasadia, R.L., Castillo, E.M., Espino, T.M., Tambalo, R.D., Padolina, W.G. , (1993) Optimization of methyl laurate preparation using immobilized lipase from Rhizopus delemar
    • Philippine Journal of Biotechnology Volume 4, No. 2
    • Ramirez, M.J.C., Tallada, Y.G., Espino, T.M., Zerrudo, J.P., del Rosario, E.J., (1994) Ligninase production by polyurethane immobilized Phanerochaete chrysosporium mutant for bleaching of abaca kraft pulp
    • Philippine Journal of Biotechnology Volume 5, No. 1
    • (1987 Je 5) Fast and Effective Diagnosis of Fruit Viruses Research Update.
    • UPLB Info. Release to Mass Media.
    • Espino, T.M., (1995) Red tide
    • University of the Philippines at Los Ba?os Volume 9, No. 1
    • Espino, R.R.C., Ligat, B.S., Magnaye, L.V., Espino, T.M. , (2000) Rehabilitation of banana bunchy top virus-Affected areas in the Philippines
    • Managing Banana and Citrus Diseases, INIBAP and FFTC

Trainings

    • USAID Training Grant on Cellulases, 1984 My-Je, North Carolina State University
    • JSPS Training on Enzyme Technology and Monoclonal Antibody Technology, 1984 Jl 9-12, Osaka University and Osaka City University, Osaka, Japan
    • JSPS Training on Monoclonal Antibody Technology, 1986 O 26-D 8, Osaka City University, Osaka, Japan
    • IDRC funded Training on Application of Monoclonal Antibodies to Virus Research, 1987 Ag 31-O 28, USDA, Beltsville, Maryland
    • JSPS Training on Enzyme Biotechnology and Monoclonal Antibody Technology, 1989 Mr 20-My 9, Osaka City University, Osaka, Japan
    • INIBAP IDRC, Workshop and Training on Banana Bunchy Top Virus, 1990 Jl 28-Ag 15, CIRAD, Cedex, France
    • The Use of Industrial Enzymes, 1991 Ja 13-19, Bangkok, Thailand
    • Mahidol University and GBF Workshop on Biotechnology of Animal Feeds and Additives, 1993 Mr 15-26, Bangkok, Thailand
    • JSPS - Visit on Monoclonal Antibody Technology and Enzyme Biotechnology, 1994 S 12-19, Osaka City University, Osaka, Japan
    • Training on the Use of Probes and PCR primers for Banana Bract Mosaic Virus Detection, 1994 O 17-27, QUT, Brisbane, Australia
    • JSPS Training on Enzyme Biotechnology, 1997 Mr 3-25, Kyoto University, Kyoto, Japan
    • PCASTRD Sponsored Seminar/Workshop on Technology Intelligence, 1998 O 5-7, Tapeyac Hotel, Baguio City
    • Development and Extension Priorities in Agriculture, Fisheries, Forestry and Natural Resources, 1999 Jl 20-21, Century Park Hotel, Malate, Manila
    • DOST-JSPS Coordinators' Meeting sponsored by the Japan Society for the Promotion of Science (JSPS), 2000 O 11-15, Indonesia
    • DOST-JSPS Coordinators' Meeting sponsored by the Japan Society for the Promotion of Science (JSPS), 2001 Mr 18-20, Bangkok, Thailand
    • The Use of Teaching Models in Genetics, 2001 Ap 23, IBS Lecture Hall 1
    • DOST-JSPS Coordinators' Meeting, 2001 Ag 22-24, Malaysia
    • Biotechnology Training Program, United States Department of Agriculture (USDA), 2001 S 8-22, Washington, D.C., USA
    • International Collaboration Workshop on Tropical Bio-resources and Green Chemistry Strategy, 2001 D 11, Traders Hotel, Pasay City
    • Training Workshop on the Safety Assessment of Genetically Modified Crops and Foods, 2002 Jl 1-3, Dusit Hotel Nikko, Makati City
    • DOST-JSPS Coordinators' Meeting of Asean Countries, 2002 D 21-24, Bangkok, Thailand
    • PCARRD National Multi-Sectoral Biotechnology Consultation Bulwagang Panday Karunungan, 2003 Mr 19, Los Baños, Laguna
    • Workshop on Priority Setting of ABSP II Projects in the Philippines, 2003 Ap 4-5, Conference Room, Makati City
    • Forum on Economic Implications of Banana and Abaca Bunchy Top Disease. Richmonde Hotel, 2003 Je 3, 21 San Miguel Avenue corner Lourdes St., Ortigas Center, Pasig City
    • DOST-JSPS Coordinators' Meeting, 2003 Jl 9-12, Kuala Lumpur, Malaysia
    • DOST-JSPS Coordinators' Meeting, 2003 D 10-15, Hanoi, Vietnam and Bangkok, Thailand
    • Symposium on Biotechnology entitled "Advances in Biotechnology Application to Coconut and Oil Palm", 2004 Ap 19-21, Manila Pavilion Hotel, United Nations Avenue, Ermita, Manila
    • Strategic Planning Workshop II, 2004 My 27-Je 16, UP Diliman, Quezon City
    • Biotechnology Information Network Planning Workshop, 2004 Jl 15, Conference Center, Pansol, Calamba City
    • NARS Needs Assessment Workshop, 2004 Jl 31-Ag 11, Santo Domingo de Heredia, Costa Rica
    • National Pest and Disease Collections in the Philippines to Underpin Agricultural Export, 2004 S 1, Splash Mountain Resort Hotel, Los Baños, Laguna
    • Commercialization of a genetically-modified (GM) Food Crop, 2004 S 14, Astoria Plaza, Ortigas Center, Pasig City
    • Abaca: Improvement of Fiber Extraction and Identification of Higher Yielding Varieties, 2004 O 19, New World Renaissance Hotel in Makati City
    • DOST-JSPS Coordinators' Meeting, Japan Society for the Promotion of Science (JSPS), 2004 D 3-4, Sanur, Bali
    • Seminar on EU Food Safety and Biotechnology, 2005 J 25, Department of Agriculture Bureau of Soils and Water Management
    • DOST-JSPS Coordinators' Meeting, 2005 F 12-18, Osaka City, Osaka, Japan
    • International Biotechnology Information Conference, 2005 O 8-15, Iowa and Nebraska, USA
    • 2nd International Health and Hygiene Symposium, 2006 F 9, DOH, Peninsula, Manila
    • 30th Asia Pacific Economic Cooperation (APEC), 2006 Mr 28-29, New World Renaissance Hotel, Makati City
    • Public Seminar on The Next Generation GM Crops: Status and Prospects, 2006 My 2, Richmonde Hotel, Pasig City
    • Biofuels Joint Undertaking of UPLB and PCARRD Operations Room, AG Samonte Building, 2006 Je 29, UP Los Baños