Scientist Profile

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Lourdes J. Cruz

National Scientist (2006), NAST Academician

Expertise: Biologically active compounds from Conus Venums:, Development of biochemical methods for monitoring paralytic shellfish poisons(PSP), Jellyfish Venoms: Biochemistry and Toxinology

Dr. Lourdes J. Cruz is recognized for her contributions to the biochemistry of conotoxins. Using the venom from Conus marine snails, her work on biologically active peptides contributed to the development of conotoxins as biochemical probes for examining the activities of the brain.

Affiliation

Professor, Marine Institute College of Science, University of the Philippines Quezon City

Research Professor, Department of Biology, Utah, U.S.A

President, Center for BioMolecular Science Foundation

President, Bataan Center for Innovative Science and Technology

Association

    • Phi Kappa Phi (University of the Philippines Chapter), Member
    • Phi Sigma (University of the Philippines Chapter), Member
    • Society of the Sigma Xi (University of the Iowa Chapter), Member
    • Philippine National Science Society, Member (Former Nat. Res. Council of the Philippines)
    • Philippine Biochemical Society, Member (Former President and Board Member)
    • International Society on Toxinology, Member
    • Society of Neuroscience, U.S.A, Member
    • International Society on Chemical Ecology, Member
    • President, Bataan Center for Innovative Science and Technology

Employment

    • International Rice Reseach Institute, Research Aide, 1962-1963
    • International Rice Research Institute, Assistant Biochemist, 1968-1969
    • Department of Chemistry, College of Medicine, University of the Philippines Manila, Assistant Professor, 1970-1973
    • Department of Chemistry, College of Medicine, University of the Philippines Manila, Associate Professor, 1973-1977
    • Department of Chemistry, College of Medicine, University of the Philippines Manila, Professor, 1977-1989
    • Department of Chemistry, College of Medicine, University of the Philippines Manila, Professor, 1977-1989
    • Department of Biology University of Utah, USA, Research Professor, 1986
    • Marine Science Institute, College of Science, University of the Philippines, Professor, 1989

Award

    • Outstanding Young Scientist Award Chemistry, National Academy of Science and Technology, 1981
    • Achievement Award Chemistry, National Research Council of the Phil., 1982
    • Eusebio Garcia Professional Chair in Biochemistry, University of the Philippines, 1985
    • Ten Outstanding Women in Nation's Service Award, Lion's Club of the Philippine and Association of TOWNS Awardees, 1987
    • Elected Member, National Academy of Science and Technology, Philippines, 1987
    • Gawad Chancellor Research Award, University of the Philippines, Diliman, 2002
    • Proclaimed as National Scientist, MalacaƱang, Manila, 10 N 2006

Research

    • Acetylcholinesterase from Conus Geographus Venom: Partial Purification and Characterization [COMPLETED]
    • Biochemical Characterization of some toxins from Conus Striatus Venom [COMPLETED]
    • Biochemical Studies on the Rabbit Cornea Opacifying Factors [COMPLETED]
    • Central Nervous System Toxins from Conus Textile Venom [COMPLETED]
    • Cornes Opacipying factors from Pseudomonas aeruginosa [COMPLETED]
    • Effects of cadang-cadang viroid infection on some nucleic acid and protein fraction of some young and mature leaves of coconut (Cocos nucifera) [COMPLETED]
    • Isolation and Characterization of Conotoxins from Conus Textile [COMPLETED]
    • Localizaton of Proteins and Enzymes in the Granules of Conus Striatus Venom [COMPLETED]
    • Preliminary Studies in some aspects of Amino Acid Biosynthesis in Juvenils of Peneanus monodon fabricius [COMPLETED]
    • Prostaglandin Synthetase from the soft coral gorgonian [COMPLETED]
    • Purification and Partial Charaterization of Nicotinamide Monucleotide Deamidase form E.Coli [COMPLETED]
    • Some Biologically Active Components of Conus quercinus Venom [COMPLETED]
    • Studies on the Biochemical Nature Conus Magus Venom [COMPLETED]
    • Studies on the Biochemical Nature of Conus Textile Venom [COMPLETED]
    • Studies on the Relationship between the Lethal component and the Rat Diaphragm paralizing the Venom from Conus Geographus [COMPLETED]
    • The Venom Duct of Conus Geographus: Some Biochemical and Histologic Studies [COMPLETED]
    • Three Paralytic Toxins from Conus Striatus Venom [COMPLETED]

Papers Presented

    • Subtype specificity properties of m-conotoxins for the voltage-gated sodium channels, 14th Philippine Chemistry Congress

Education

    • University of the Philippines, Diliman, Quezon City, Philippines, B.S. Chemistry, 1962
    • University of Iowa, USA, M.S. in Biochemistry, 1966
    • University of Iowa, USA, Ph.D. in Biochemestry, 1968

Publication

    • Clark, C. , Olivera B.M., Cruz L.J., (1981) A toxin from Conus geography venom which acts on the vertebrate central nervous system
    • Toxicon Volume 19
    • Buenaflor, H.G., Mendoza, E.M.T. Cruz, L.J., (1981) Studies of the biochemical nature of Conus magus venom
    • Kalikasan, Phil. J. Biol.Soc. Volume 10
    • Jimenez, E.C Cruz, L.J., (1981) Localization of ezymes and protiens in the venom granules of Conus striadius
    • Bull.Phil. Biochem.Soc. Volume 4
    • Mclnstosh, M. Cruz, L.J. Hunkapiller M.W., Hood, L.E., Luque, A., Gray, W., Oliver, B.M., (1982) A peptide toxin from conus magus venom
    • Arch. Biochem. Biophys. Volume 218
    • Jimenez, E.C., Olivera, B.M., Cruz, L.J., (1983) Localization of enzymes and possible toxin precursors in granules form Conus stiatus venom
    • Toxicon supplement Volume 3
    • Cruz, L.J, (1983) Biochemical charaterization of toxin from Conus striatus venom.NRCP
    • Saligang Saliksik Volume 11
    • Reyes, A.G., Cruz, L.J., (1983) Hemolytic activities from Conus textiles venom
    • Acta Medica Philipina Volume 19
    • Lirazan, M.B., Cruz,L.J., (1982) Biochemical characterization of some toxins from Conus striatus venom
    • Bull. Phil. Biochem.Sco. Volume 5-6
    • Gray, W.R., Rivier, J.E., Galyean, R., Cruz, L.J, Olivera, B.M., (1983) Conotoxin M1: Dissulfide bonding and conformation states.
    • J. Biol.Chem. Volume 258
    • Gray, W.R., Luque, T.A.., Galyean, R., Atherton, E., Sheppard, R.C., Stone, B.L., Reyes, A., Alfrod, J., Mclntosh, M., Oliver, B.M.,Cruz, L.J., Riveir, J., (1984) Conotoxin G1: Disulfide brides, synthesis and preparation of iodinated derivatives
    • Biochemistry Volume 23
    • Olivera, B.M. Mclntosh, J.M. Cruz, L.J., Luque, F.A., Gray, W.R., (1984) Purification and sequence of a presynaptic peptide toxin from Conus geography venom
    • Biochemistry Volume 23
    • Mclntosh, J.M., Olivera, B.M., Cruz, L.J., Gray, W.R., (1984) carboxyglutamate in a neuroactive toxin
    • J.Biol. Chem. Volume 259
    • Olivera, B.M., Mclntosh, J.M. Clark C., Middlemas, D., Gray W.R ., and Cruz L.J., (1985) a sleep-inducing peptide from Conus geographus venom.
    • Toxicon Volume 23
    • Cruz, L..J,, Gray W.R., Olivera B.M , Zeikus R.D., Kerr L., Yoshikami, D., and Moczydlowski E., (1985) Conus geograhus toxins that descriminate between neuronal and muscle sodium channels
    • Journal Biology Chemistry Volume 260;
    • Gray W.R., Olivera B.M, Cruz L.J., and Rivier, J., (1987) A model for " sleepers peptide (conotoxin GV) and other Gla- containing molecules.Peptide Chemistry, T. Shiba and S. Sakakibara (eds.)
    • Protein Research Foundation, Osaka Japan
    • Cruz, L.J., Johnson, D.S., Imperial, J.S., Griffin, D LeCheminant G.W., Miljanich,G.P., Olivera B.M., (1987) Conotoxin and voltage- sensitivity Ca channel subtypes
    • Molecular Biology - IOnic channels. Current topics in Membranes and Transports Volume 3
    • Olivera, B.M., Gray, W.R., Cruz L..J.,, (1988) Marine snail venom.
    • Handbook of Natural Toxins, Vol 3, Marine toxins and Venoms, A Tu (ed.) Marcel Dekker, Inc., N.Y., Volume 3
    • Gray, W.R., Olivera B.M., Cruz L.J.,, (1988) Peptide toxins from venomous Conus snails
    • Ann. Rev. Biochemistry Volume 57
    • Zafaralla, G.C. Camilo, C.A., Gray, W.R., Karlstrom, R., Olivera, B.M., and Cruz, LJ., (1988) Phytogenetic specificity of chlonergenic ligands: - Conotoxin SI.
    • Biochemistry Volume 27
    • Johnson, D.S., Corpuz,G.P., and Cruz, L..,, (1988) Conotoxins as probes for subtypes of neuronal calcium channels.
    • Proceedings International Symposium on Protein Structure and Function Relationship
    • Lecheminant, G.W., Reyes, A.G., and Cruz, L. G., (1989) Conotoxins binding: An assay for muscle subtypes of voltage- sensitivity sodium channels
    • Transaction National Academy Science & Technology Volume 10
    • Cruz, L.J., Kupryszewski, Lechiminant, G.W., Gray , W.R., Olivera, B.M., AND Cruz, L.J., (1989) Conotoxin GIIIA, a peptide ligand for muscle Ma channels: Chemical synthesis, radiolabeling and receptor characterization.
    • Biochemistry Volume 28
    • Hillyard, R.D., Olivera, B.M., Woodward, S., Corpuz, J.P., Gray, W.R., Ramilo, C.A., and Cruz, L.J., (1989) A molluscivorous Conus toxin:conserved frameworks in conotoxins.
    • Biochemistry Volume 28
    • Cruz, L.J.,, (1989) Conotoxins: Biochemical probes for ion channels and receptors.
    • Natural Toxin Characterization, Pharmacology and Therapeutics.
    • Haack, J.A., Rivier, J., Parks, T.N., Mena, E., Cruz, L.J., Olivera, B.M., (1990) Conantocxins-T; a y- carboxyglutumate-containing pepetide with N-methyl-D-aspartate antagonist activity.
    • Journal Biology Chemistry Volume 265
    • Olivera, B.M., Hillyard, D.R., River, J., Woodward, S., Gray, W.R., Corpuz, G.P., and Cruz, L.J.,, (1990) Conotoxins: Targeted peptide ligands fron snail venom.
    • Marine Toxins: Origin, Structure , and Molecular Pharmacology. S. Hall and G. Strichartz (eds.) , ACS Syposium Series, American Chemical Society, Washington, D.C
    • Cruz, L.J, Myers, R.A., Haack, J.A., and Olivera B.M., (1990) Toxins and other biologically active peptides of Conus geographus venom.
    • Proc. 2nd EurAsia Conference in Chemistry , Seuol, Korea,
    • Olivera, B.M., Gray, W.R., Zeikus, R., Mclntosh, J.M., Varga, J., Rivier, J., De Santos, V., Cruz, L.J., (1985) Peptide neurotoxins from fish-hunting cone snails
    • Scince Volume 230
    • Cruz, L.J., Gray, W., Yoshikami, D., Oliver, B.M.,, (1985) Conus venoms: A rich source of neuroactive peptides
    • Journal Toxicology-Toxin Review Volume 4
    • Gray, W.R., Middlemas, D.M., Zeikus, R., Olivera, B. , Cruz, L.J., (1985) Structure-activity relatioship in conotoxins: A model ln : Peptides: Structure and Function, Pronc. Ninth
    • Amer.Peptides System
    • Cruz, L.J., Olivera, B.M., (1986) Calcuim channel antagonist,- Conotoxin defines a new high affinity site.
    • J.Bio.Chem. Volume 261
    • Rivier, J., Galyean,R., Gray, W.R., Azim - Zonooz, A., Mclntosh, J.M., Cruz, L.J, Oliver, B.M.,, (1987) Neuronal calcium channel inhibitor, Synthesis of -conotoxin in GVIA and effects on 45Ca uptake by synotosomes
    • Journal Biology Chemistry Volume 262
    • Balbin-Romero, J., Cruz, J.L., (1987) Actycholinesierase of Conus geography venom: Partial purification and characterizxation
    • Acta Medica Phili. Volume 7
    • Conception, G.P., Cruz, L.J., (1987) Prostaglaadin synthetase from Philippines gorgonians
    • Bull. Phil. BioChem.Soc. Volume 7
    • Olivera, B.M., Cruz, L.J., Santos De,. V., LeCheminant, G., Griffin, D., Zeikus, R., Mclntosh, J.M., Galyean R., Varga, J., Gray, W.R., Rivier, J., (1987) Neuronal Ca channel antagonists. Discrimination between Ca channel subtypes using - conotoxin from Conus magus venom
    • Biochemistry Volume 26
    • Cruz, L.J., Jonhson, D.S, Olivera, B.M.,, (1987) A characterization of the conotoxin target, Evidence for tissue -spectific heterogeneity in Ca channel types
    • Biochemistry Volume 26
    • Mc Cleskey, E.W., Fox, A.P., Feldman, D., Cruz, L.J., Olivera, B.M., Tsien, R.W., Yoshikami, (1987) Calcium channel blockade by a peptide from Conus specificity and mechanism
    • Proceedings, National Academy of Science, U.S.A Volume 84
    • Yeager, R.E., Yoshikami, J., Rivier,J., Cruz, L.J., Mijanich, G.P, (1987) Transmitter release from presynaptic terminals of the electric organ : Inhibition by the calcium channel antagonist,- Conus toxin
    • Journal of Neuroscience Volume 7
    • Cruz, L.J., Lecheminant , G.W., Olivera, B.M., (1987) Biochemical studies of conotoxin GVIA; A peptide toxin inhibiting voltage -sensitive Ca++ channels in.
    • Integration and control of metabolic processes, pure and applied aspects.
    • Cruz, L.J., De Santos, V., Zarafalla , G.C., Ramilo, C. A., Ziekus, R., Gray, W.R., Olivera, B.M., (1987) Invertebrate vasopressin/oxytocin homologs, characterization of peptides from conus geographus and conus striatus venoms.
    • J. Biol. chem. Volume 262
    • Rivier, J., Galyean, R..,Simon, L. Cruz, L.J., Olivera, B.M. Gray, W.R., (1987) Total synthesis and further characterization of the carboxyglutamate-containng "sleeper" peptide from conus geographus venon.
    • Biochemistry Volume 26
    • Woodward, S.R., Cruz, LJ.,Olivera, B.M.,Hillyard, D.R., (1990) Constant and hypervariable region in conotoxin properties.
    • Embo Journal Volume 9
    • Olivera, B.M., Rivier, J., Clark., C., Ramillo., C.A., Corpuz., G.P., Abogadie., F.C., Mena., Woodward., S.R., Hillyard., D.R., Cruz., L.J., (1990) Diversity of conus neuropeptides
    • Science Volume 249
    • Mena., E.E., Gullak., M.F., Pagnozzi., M.J., Richter., K.E., Cruz., L.J., Olivera., E.E., (1990) Conatoin -G: a novel peptide antagonist to the n-methyl-D-aspartic acid (NMDA) receptor.
    • Neuroscience letters Volume 118
    • Myers, L.J., Mcintosh, J.M., Imperial, J.S., Williams, R.W. Oas, T. Haack, J.A., Fernandes, J.F., Rivier, J. Cruz, L.J., Olivera, B.M., (1990) Peptides from conus venoms which affect Ca++ entry into neurons.
    • J. Toxicol.-Toxin Reviews Volume 9
    • Bautista, G.M. , Lugay, J.C. Cruz, L.J. Juliano, B.O., (1964) Glutamic acid decaboxylase activity as viability index artificially dried and stored rice.
    • Cereal Chemistry Volume 41
    • Juliano, B.O., G.B., Cagampang, G.B. , L.G. Cruz, L.J. Santiago, R.S., (1964) Some physicochemical properties of rice in Southeast Asia.
    • Cereal Chemistry Volume 41
    • Cagampang, G.B., Cruz, L.J. Espiritu, S.G. Santiago, R.S., (1968) Studies on the extraction and composition of rice proteins
    • Cereal Chemistry Volume 43
    • Cruz, L.G., L.B. Glense, and C.P. Berg, (1969) Mutual antagonism in the metabolism of D- valine and D-leucine and antagonism by their analogos.
    • Cereal Chemistry Volume 136
    • Cagampang, G.B. ,Cruz, L.J. Juliano, B.O., (1971) Free amino acid in the bleeding sap and the developing rice grain of the rice plant.
    • Cereal Chemistry Volume 48
    • Cruz, L.J. Salabao, E. Flores, and B.M. Olivera, (1975) Pyridine nucleotide metabolism: Purification of a nicotinamide-specific deamidase
    • Kimikal Volume 1
    • Cruz, L.J. Reyes, A. Corpuz, G. Tecson, E.M. Olivera, B.M., (1975) A study of Conos venom proteins.
    • NRCP Research Bull Volume 31
    • Cruz, L.J. Corpuz, G. Olivera, B.M., (1976) A preliminary study of Conos venom protein
    • Veliger Volume 18
    • Nacino, F.M. Baretto, C.B. Cruz, L.J., (1975) The effect of citrus oil on tumor production by DMBA.
    • Kalikasan, Journal of Biochemistry Volume 4
    • Cruz, L.J., (1977) Quantitative assay for Conus toxin
    • Acta Medica Philipina Volume 13
    • Cruz, L.J., Corpuz, G. Olivera, B.M., (1977) Mating, spawning, development and feeding habits of geographus in captivity.
    • Nautilus Volume 92
    • Pali, E.S. Tango, O.M. Cruz, L.J., (1979) The venom duct of Conis geographus:Some biochemical and hetologic studies
    • Bulletin of Philippine Biochemical Society Volume 2
    • Cruz, L.J. . Gray, W.R. Olivera, B.M., (1978) Purification and properties of a mytoxin from Conus geographus
    • Archives of Biochemistry Biophysics. Volume 190
    • Olivera, B.M., Rechstenier, M.C. Ramos, P.M. Curz, L.J. Hillyard, D., (1980) ADP ribosy lation and the pyride nucleotide cylce
    • Ribosylation of Regulary Enzymes and Protiens
    • Coloso, R.M., Cruz, L.J., (1980) Preliminary studies in some aspects of amino acid biosynthesis in juveniles of Penaeaus monodon fabicus I. Incoporation of 14c from (U-14 c)-acetate in to amino acid of precipitable proyiens
    • Bulletin of Philippine Biochemical Society Volume 3
    • Coloso, R.M. Cruz, L.J., (1980) Preliminary studies in some aspects of amino acid biosynthesis in juveniles of Penaeaus monodon fabicus II. Partial purification and characterization of muscle-L- glutamate dehydrogenase.
    • Bulletin of Philippine Biochemical Society Volume 3
    • Hillard, D., Rechestenier, M. Ramos, P.M. Imperial, L.J. Olivera B.M., (1981) The pryridine nucleotide cycle
    • Studies in Escherichia coli and the human cell line D98/AH2. Volume 256
    • Cruz, L.J., (1985) Biochemical characterization of some toxins from Conus Striatus Venom.
    • Bulletin of Philippine Biochemical Society Volume 5, No. 6
    • Cruz, L. J., () Conus geographus toxins that discriminate between neuronal and sodium channels.
    • Journal of Biological Chemistry Volume 260
    • Cruz, L.J., () Peptide toxins from Conud geographus venom.
    • Toxicon. Volume 23
    • Cruz, L.J., () Studies on the biochemical nature of Conus magus venom
    • Kalikasan, Philippine Journal of Biology Volume 10
    • Cruz, L.J. Reyes, A., () Conotoxin in GI: Disulfide bridges, synthesis and preparation of iodinated derivative.
    • Biological Chemistry Volume 258
    • Lirazan, M.B. Grey Craig, A. Shetty R. Walker, C.S. Olivera, B.M. Cruz, L.J., (1999) Multiple bromotryptophan and y-carboxyglutamate residues in a Conus peptide.
    • Philippine Journal of Science Volume 128, No. (3):
    • Monje, V,D. Ward, R. Olivera, B.M. Cruz, L.J., (1999) 16S mitochondrial ribosomal RNA gene sequences: A comparison od seven Conus species.
    • Philippine Journal of Science Volume 128, No. (3):
    • Lirazan, M. Jimenez, E.C. Grey Craig, A. Olivera, B.M. Cruz, L.J., (2002) Conophysin-R, a Conus radiatus venom peptide belonging to the neurophysin family.
    • Toxicon Volume 40
    • Jimenez, E.C. Donevan, S. Walker, C. Zhou, L.M. Nielsen, J. Cruz, L.J. Armstrong, H. White, H.S. Olivera, B.M., (2002) Conantokin-L, a new NMDA receptor antagonist.: determinants for anticonvulsant potency.
    • Epilepsy Research Volume 51
    • Espiritu, J.D. Cruz, L.J. Cartier, G.E. Olivera, B.M., (2002) Venomous gastropods: Conus, conoideans and other neogastropod families.
    • Supp.4, Systematics Phylogeney and Biology of the Neogastropoda, 3rd International Workshop on Malacology, Bolletino Malacologico Volume 38;
    • Ontengco, D.C. Talaue, M. Cruz, L.J. Capal, T.V., (1999) The MICs of betel oil against common clinical pathogens.
    • Acta Manilana Volume 47
    • Floresca, MC.Z. Cruz, L. J., (1998) Subtype specificity properties of m-conotoxins for the voltage-gated sodium channels.
    • Proc. of the 14th Philippine Chemistry Congress

Trainings

    • Postdoctroral training, 1970 - 1970, Department of Biology, Kansas State University, U.S.A
    • Postdoctoral training, 1976 - present, Department of Biology University of Utah,U.S.A