Mr . Kamantigue is one of the S&T Fellows in DOST-FPRDI. He is currently taking his Doctorate degree in Biochemistry from the University of the Philippines Manila. His research interests are Plant Chemistry and Pharmaceutical Biostatistics and Plant Chemistry

Sex: Male
Education:

• Doctor of Biochemistry, University of the Philippines Manila, ongoing
• Master of Science in Pharmacy major in Pharmaceutical Chemistry, University of the Philippines Manila, 2017
• Bachelor of Science in Pharmacy, Our Lady of Fatima University, 2011

Field of Specialization:
Plant Chemistry
Pharmaceutical Biostatistics and Plant Chemistry

Researches:

Article title: In vitro mammalian alpha-glucosidase inhibitor screening of selected plant materials from Siba-o, Calabanga, Camarines Sur.
Authors: Edmark C. Kamantigue, Judilynn S. Solidum, Noel S. Quiming, Marilou G. Nicolas, Switzale M. Pidlaon
Publication title: Philippine Journal Health Science and Research 21(3), 2017

Abstract:
Background and Objectives: Diabetes mellitus type 2 (DM2) remains a health threat to Filipinos. According to the International Diabetes Federation 2014, the Philippines is one of the emerging DM2 hotspots with an estimated prevalence rate of around 3.2 million cases (5.9%) between 20 to 79 years old. In line with the acceptance of Filipino patients with the utilization of herbal medicine as an effective alternative for treatment of their ailments, some of the selected plant materials from Siba-o, Calabanga, Camarines Sur were explored for mammalian intestinal alpha-glucosidase inhibition in vitro to develop new herbal drug candidates that are effective, safe, and more affordable.

Methodology: Exhaustive maceration using absolute ethanol was performed to extract the phytoconstituents from the plant matrix. In vitro alpha-glucosidase inhibition assay using spectroscopic method (96 well plates) was carried out to analyze the mammalian α-glucosidase inhibition of the different plant samples, IC₅₀ was determined from the generated linear regression extrapolated from concentrations-% inhibitions plot. Thin Layer Chromatography (TLC) bioautography was employed to identify the presence of flavonoids, tannins, essential oil, reducing sugar, coumarin, anthraquinones, anthrones, steroids, alkaloids, and peptides.

Results: From the 98 crude plant samples extracted, the ethanolic extracts of Melothria sp. stem with leaves showed a concentration-dependent inhibition activity towards mammalian α-glucosidase from rat intestine acetone powder with IC₅₀ values of 49.24 ppm. Tannins, flavonoids, essential oils, and indoles were detected from TLC bioautography that may be responsible for the bioactivity.

Conclusions and Recommendations: The results demonstrated the potential utilization of some plant samples as an alternative herbal drug. However, only Melothria sp. crude leaves and stem extract (SB32LS) showed a concentration-dependent activity and further studies must be done to isolate the metabolites responsible for the activity through activity-guided isolation.
Full text available upon request to the author

Article title: Partial purification of the mammalian alpha-glucosidase inhibitor from Melothria sp. (Fam. Cucurbitaceae) leaves and stem extract: in vitro.
Authors: Edmark C. Kamantigue, Noel S. Quiming, Judylynn N. Solidum, and Marilou G. Nicolas
Publication title: JSTAR, Special Edition 2019

Abstract:
Diabetes Mellitus Type 2 (DM2) is a chronic disease characterized by insufficient insulin levels, pancreatic beta cells function loss and insulin resistance in peripheral tissue. Voglibose and acarbose are the clinically used alpha-glucosidase inhibitor; however, adverse effects such as nausea, diarrhea, and bloating hinder the utilization of these agents. Hence, there is a need to explore for an alternative drug that has fewer side effects, better activity, and more affordable than the current commercial drugs. In this study, Melothria sp. was purified using normal phase column chromatography. Seven fractions (fraction A-G) were separated and tested for mammalian alpha-glucosidase assay in vitro and phytochemical screening was conducted to determine the metabolites present in each fraction. Fraction F showed a promising activity against the enzyme and comparable to other natural products and commercial alphaglucosidase agent, acarbose. Phenolic compounds such as tannins and flavonoids based on the phytochemical screening were detected present in the sample which can be responsible in the inhibition activity of the semi-purified extracts.
Full text available upon request to the author

Article title: In Silico screening of selected Natural Product Compounds against Human Neutrophil Elastase.
Authors: Johnalyn C. Go and Edmark C. Kamantigue
Publication title: JSTAR, Special Edition, 2019

Abstract:
Human neutrophil elastase (HNE) is one of the key proteases present in the neutrophil and is involved in the pathogenesis of different inflammatory disorders. In recent years, the investigation for potential HNE inhibitors are increasing and one of the strategies in drug discovery is the use of in silico drug screening of the reported isolated compounds from natural products. In this study, the docking behavior of HNE with a series of compounds isolated from herbs that inhibit lung inflammation was investigated. iGEMDOCK v.2.1. (Graphical Environment for Recognizing Pharmacological Interactions and Virtual Screening) was used for the docking, virtual screening, and post-screening analysis of pharmacological interactions between the enzyme HNE and various lead compounds as the ligand. And based on the results, Compound 22 ([(2R,3R, 4S, 6S0-3,4,5- trihydroxy-6{[(2R)-5-hydroxy-2-(4-hydroxyphenyl0-4-oxo-2,3- dihydro-1-benzopyran-7-yl]oxy}oxan-2-yl]methyl(2E)-3-4- hydroxyphenyl)prop-2-enoate has the highest inhibition potential according to the calculated Gibbs free energy. The finding of this study is the first to be reported based on the current knowledge of the authors. It is recommended that further analysis of the test compound must be performed both in vitro and in vivo to validate its bioactivity.
Full text available upon request to the author

Article title: Perception on, Propagation and Usage of Medicinal Plants Among Selected Barangays in Sampaloc, Manila, Philippines.
Authors: Edmark Kamantigue, Angelica Balais-Rin, Marilen Pacis, and Fritz Gerald V. Jabonete
Publication title: Journal of Sciences, Arts, and Technology Research. Vol 6, Sept 2020

Abstract:
Medicinal plants are widely used in the Philippines particularly in rural areas as an alternative to synthetic drugs. However, there have been limited studies about the usage of medicinal plants in urban areas. The aim was to determine the perception of, propagation, and usage of medicinal plants in two partner barangays in Sampaloc, Manila. A convenience sampling was utilized in the study. The researchers utilized a questionnaire to survey (available in English and Filipino language) on the awareness and knowledge about the utilization, preparation, cultivation, and propagation of medicinal plants of the respondents. A group semi-structured interview was utilized to validate the answers of the respondents. This was done in two partner barangays in Sampaloc, Manila. The data were analyzed using the Informant consensus factor (ICF), Use value (UV), and Relative frequency citation (RFC). The data collection was conducted from October to November 2019. Both partner barangays perceived that the synthetic agent is still their primary option in treating their ailments due to established safety and efficacy. Furthermore, the partner barangays were not equipped with the basic knowledge in the proper usage of herbal plants in the community herbal garden, and herbal plants were used as adjuvant or supportive treatment. This study showed that both adopted barangays utilized medicinal plants as an adjuvant in their synthetic medication due to its claimed safety and medicinal plants. The most propagated and cultivated medicinal plant is oregano used as a treatment for asthma and cough was the well-known plant while banaba, sabila, gugo, and tanglad were the least. It is recommended that information dissemination campaigns especially on the DOH-approved medicinal plants be strengthened and heightened to increase participation from the barangay counterparts.
Full text available upon request to the author

Article title: Chemical Derivatization-based UV-Vis Spectrometry Analysis of Aminophenol Metabolites in a Biological Matrix.
Authors: Go, J., Kamantigue, E., and Orejola, J.J.
Publication title: Journal of Sciences, Arts, and Technology Research 2022

Abstract:
No abstract
Full text available upon request to the author

Article title: Direct TLC-Bioautography guided fractionation of free radical scavenging activity of Barringtonia racemosa L. (Fam:Lecythidaceae) leaf non-polar fraction.
Authors: Kamantigue, E., Quiming, N, Solidum, J., and Nicolas, M.
Publication title: Journal of Sciences, Arts, and Technology Research. 2022

Abstract:
No abstract
Full text available upon request to the author

Papers Presented:

Article title: In silico screening of potential anti-aging agent through CYP2D6 inhibition of selected isolated natural products.
Authors: Kamantigue, E.
Conference title: 2nd Faculty Research Colloquium, Multipurpose Hall, Annex Building, National U, Manila, Sept. 23, 2019

Article title: In Vitro Porcine Pancreatic Alpha-Amylase Inhibition of Crude Extract and In Silico Study of Selected Compounds Isolated from Lagestoemia speciosa (Lythraceae) leaves
Authors: Kamantigue, E. et al
Conference title: IUBMB Education Conference 2019 and 46th PSBMB Annual Convention, Manila Hotel, Manila City, Philippines.

Article title: Mammalian alpha-glucosidase inhibition activity of non-tannin fraction of (Melothria ap. Fam. Cucuritaceae) leaves and stem extract”
Authors: Kamantigue, E., Quiming, N, Solidum, J., and Nicolas, M.
Conference title: IUBMB Education Conference 2019 and 46th PSBMB Annual Convention, Manila Hotel, Manila City, Philippines.

Article title: In Silico Screening of Compounds with Insect Acetylcholinesterase Inhibitory Activity
Authors: Pangilinan, C.J., Go, J., and Kamantigue, E.
Conference title: IUBMB Education Conference 2019 and 46th PSBMB Annual Convention, Manila Hotel, Manila City, Philippines.

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