Sex: Male
Education:
- University of the Philippines Los Baños
Field of Specialization:
Mangoes
Biotechnology
Researches:
Article title: Nutrient Composition, Antioxidant and Antibacterial Activities of Ulva prolifera O. F. Müller
Authors: Eldrin De Los Reyes Arguelles and Arsenia B Sapin
Publication title: Squalen Bulletin 2021
Abstract:
Seaweeds are marine organisms capable of producing diverse kinds of chemical compounds with promising pharmacological use. The study evaluated the proximate and elemental composition and the potential antioxidant (using CUPRAC, ABTS+, and DPPH assays) and antibacterial activities (using microtiter plate dilution assay) of Ulva prolifera O. F. Müller. The seaweed has a total phenolic content of 829 ± 2.00 g GAE/g. Antioxidant efficiency of U. prolifera exerted high ability of reducing copper ions, potent ABTS+ and DPPH scavenging activities in a concentration-dependent manner with IC50 value of 24.7 g GAE/mL, 43.52 g GAE/mL, and 54.1 g GAE/mL, respectively, more effective than ascorbic acid. In vitro antibacterial activity assay showed that U. prolifera exhibited inhibitory activity against bacterial pathogens such as Methicillin-resistant Staphylococcus aureus (MIC = 125 g/mL), S. epidermidis (MIC = 125 g/mL), and Pseudomonas fluorescens (MIC = 500 g/mL). This investigation is the first documented report regarding antibacterial activity of U. prolifera against P. fluorescens. In addition, results showed elemental composition to be in decreasing order of Ca > K > Mg > Na > Mn > Fe > Zn > Cu > Pb > Cd > Cr. Proximate composition of U. prolifera showed high carbohydrate and protein content with a percentage composition of 36.20 ± 0.27% and 23.72 ± 0.31% (dry weight), respectively. This study is the first report in the Philippines that shows the potential of U. prolifera as an excellent candidate organism as source of chemical compounds with relevant application to the pharmacological industry.
Full text link https://tinyurl.com/3bj6v8zx
Article title: Chemical Composition and In vitro Antioxidant and Antibacterial Properties of the Edible Cyanobacterium, Nostoc commune
Authors: Milagrosa R. Martinez-Goss, Eldrin DLR. Arguelles, Arsenia B. Sapin, and Ronaniel A. Almeda
Publication title: Philippine Science Letters 14(supplement), 2021
Abstract:
The nutritional composition of the edible cyanobacterium, Nostoc commune Vaucher, was evaluated, including its potential antioxidant and antibacterial properties. Results showed that the elemental composition were in decreasing order as: Ca > Fe > Mg > Na > K > Mn > Zn > Cu > Cr > Pd > Cd. Proximate composition of N. commune showed high carbohydrate and protein content with estimated value of 65.27 ± 0.09% and 21.51± 0.06%, respectively. The Nostoc commune (NC) methanolic crude extract had an average total phenolic content of 88.2 mg, as gallic acid equivalent (GAE)/ 100 g, and a mean value of 17.45 mg as quercetin equivalent/100 g for total flavonoids. Relative antioxidant efficiency of N. commune exerted potent copper reducing activity with IC50 value of 10.84 ± 0.015 μg GAE/mL. The tested algal extract exhibited antioxidant activity that is dose-dependent and positively correlated to its phenolic content. Acidified methanolic extract of N. commune showed an extended spectrum of inhibitory activity against Gram-positive bacteria, Staphylococcus aureus, Staphylococcus epidermidis and Bacillus cereus with MIC (minimum inhibitory concentration) of 125 and 250 μg/mL, respectively. Also, it is moderately active against Bacillus cereus with MIC and MBC (minimum bactrericidal concentration) of 500 and 1000 μg/mL. This study is useful in the context of improved utilization of N. commune as alternative source of functional products possessing multi-bioactivities with potential use in food and pharmaceutical industries, being not only economically advantageous but also environmentally friendly.
Full text link https://tinyurl.com/yebrwvza
Article title: Chemical Composition and Bioactive Properties of Sargassum aquifolium (Turner) C. Agardh and Its Potential for Pharmaceutical Application
Authors: Eldrin DLR. Arguelles and Arsenia B. Sapin
Publication title: Philippine Journal of Science 151(S1):9-24, August 2021
Abstract:
Seaweeds are known sources of marine bioactive substances with diverse biological activities important in the synthesis of medically important novel drugs. The proximate and elemental compositions as well as bioactive properties of a brown macroalga, Sargassum aquifolium (Turner) C. Agardh, were studied. Results showed that proximate composition of S. aquifolium contain high carbohydrate (32.29 ± 0.17%) and ash (30.19 ± 0.14%) content. Elemental composition of the seaweed exhibited a decreasing order of Na > Ca > K > Mg > Mn > Fe > Zn > Cu > Pb > Cr > Cd. The seaweed had a total phenolic content (TPC) of 5.74 ± 0.04 mg GAE/g. Antioxidant activities of S. aquifolium were characterized by having potent ABTS + [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)] scavenging activity and high copper reduction capacity with IC 50 value of 107 μg/mL and 21.01 μg/mL respectively. Evaluation of tyrosinase and elastase inhibition activities showed that S. aquifolium extract has potent inhibition activities with IC 50 of 39.00 μg/mL and IC 50 of 231.00 μg/mL, respectively-more effective than kojic acid and tocopherol. In addition, in vitro assessment of α-glucosidase and α-amylase inhibition property showed that S. aquifolium extract has potent inhibitory activity as compared to acarbose (standard anti-diabetic drug) with IC 50 of 15.60 and 59.0 μg/mL, respectively. Also, the S. aquifolium extract exhibited effective antimicrobial activities against bacterial pathogens such as penicillin acylase-producing Bacillus cereus (MIC = 125 μg/mL), Staphylococcus saprophyticus (MIC = 250 μg/mL), methicillin-resistant Staphylococcus aureus (MRSA) (MIC = 250 μg/mL), Staphylococcus epidermidis (MIC = 250 μg/ mL), and Pseudomonas aeruginosa (MIC = 500 μg/mL). The current investigation is a pioneering study in the Philippines that shows the potential of S. aquifolium as novel source of bioactive compounds with important use for pharmaceutical applications.
Full text link https://tinyurl.com/7jppxrfa
Article title: Bioprospecting of Turbinaria ornata (Fucales,Phaeophyceae) for cosmetic application: antioxidant, tyrosinase inhibition and antibacterial activities
Authors: Eldrin DLR. Arguelles and Arsenia B. Sapin
Publication title: Journal of the International Society for Southeast Asian Agricultural Sciences 26(2):30-41
Abstract:
Brown macroalgae are rich sources of polyphenols with diverse pharmacological activities relevant to formulation of novel cosmetic products. This study evaluated the antioxidant, tyrosinase inhibition, and antibacterial properties of a brown macroalga, Turbinaria ornata (Turner) J. Agardh. The seaweed extract had a total phenolic content of 18.50 ± 0.17 mg GAE/g. Antioxidant efficiency of T. ornata exerted high copper reduction antioxidant capacity and potent DPPH radical scavenging activity in a dose-dependent manner with EC50 value of 24.34 μg GAE/mL and 22.10 μg GAE/mL, respectively showing its potential as an alternative source of effective natural antioxidant. T. ornata has an excellent tyrosinase inhibitory activity (EC50 of 67.50 μg GAE/mL), more effective than that of commercially available skin-lightening ingredient, kojic acid with EC50 of 109.8 μg/mL. In vitro antibacterial activity using microdilution technique assay demonstrated T. ornata possessed strong inhibitory activity against common bacterial skin pathogen such as Staphylococcus aureus (MIC = 125 μg/mL), Staphylococcus saprophyticus (MIC = 125 μg/mL), Staphylococcus epidermidis (MIC = 250 μg/mL) and Serratia marcescens (MIC = 500 μg/mL). The current study is the first report in the Philippines exploring the biological properties of T. ornata as potential alternative source of skin lightening active ingredient important for cosmetic application.
Full text link https://tinyurl.com/dwhrdpms
Article title: In vitro antioxidant, alpha-glucosidase inhibition and antibacterial properties of Turbinaria decurrens Bory (Sargassaceae, Ochrophyta)
Authors: Eldrin DLR. Arguelles, Arsenia B. Sapin
Publication title: Asia Pacific Journal of Science and Technology 25(3), September 2020
Abstract:
The genus Turbinaria is a chemically diverse group of seaweeds containing several phytochemicals with unique pharmacological activities useful in the treatment and management of diseases. This study was done to evaluate the bioactive potential of a brown macroalga, Turbinaria decurrens Bory from Pagkalitan, Batangas. The acidified methanolic extract of T. decurrens has a total phenolic content of 27.84 ± 0.12 mg gallic acid equivalents (GAE)/g. Antioxidant efficacy of T. decurrens exerted potent radical scavenging activity against 2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2-diphenyl-1-picrylhydrazyl (DPPH) free radicals as well as high reducing capability of copper ions in a dose-dependent manner with half maximal inhibitory concentration (IC50) value of 49.31 μg GAE/mL, 69.91 μg GAE/mL and 17.2 μg GAE/mL, respectively. In vitro study of α-glucosidase inhibition showed that T. decurrens has potent inhibitory activity (IC50 of 11 μg/mL) as compared to that of acarbose and metformin (anti-diabetic drugs) with IC50 values of 7960 μg/mL and 49.66 mg/mL, respectively. Assessment of the antibacterial activities using microtiter plate dilution assay showed that T. decurrens have strong activity against Methicillin-resistant Staphylococcus aureus, minimum inhibitory concentration (MIC=125 μg/mL) followed by Penicillin-acylase producing Bacillus cereus (MIC=250 μg/mL), Staphylococcus epidermidis (MIC=250 μg/mL), and Pseudomonas aeruginosa (MIC=500 μg/mL). This investigation is one of the pioneering research studies in the Philippines showing the potential of T. decurrens extract as a cheap source of biologically active compounds with potential use for the treatment of drug-resistant bacterial infection and diabetes.
Full text link https://tinyurl.com/tncjd2eh
Article title: Bioactive properties of Sargassum siliquosum J. Agardh (Fucales, Ochrophyta) and its potential as source of skin-lightening active ingredient for cosmetic application
Authors: Eldrin DLR. Arguelles, Arsenia B. Sapin
Publication title: Journal of Applied Pharmaceutical Science 10(7):51-058, July 2020
Abstract:
Seaweeds are notable in producing diverse kinds of polyphenolic compounds with direct relevance to cosmetic application. This investigation was done to assess the bioactive properties of a brown macroalga, Sargassum siliquosum J. Agardh. The alga has a total phenolic content of 30.34 ± 0.00 mg gallic acid equivalents (GAE) g −1. Relative antioxidant efficiency showed that S. siliquosum exerted a potent diphenyl-1, 2-picrylhydrazyl scavenging activity and high ability of reducing copper ions in a dose-dependent manner with an IC 50 value of 0.19 mg GAE ml −1 and 18.50 μg GAE ml −1 , respectively. Evaluation of antibacterial activities using microtiter plate dilution assay revealed that S. siliquosum showed a strong activity against bacterial skin pathogen, Staphylococcus aureus (minimum inhibitory concentration (MIC) = 125 µg ml −1 and minimum bactericidal concentration (MBC) = 250 μg ml −1) and Staphylococcus epidermidis (MIC = 250 µg ml −1 and MBC = 500 μg ml −1). In vitro study of tyrosinase inhibition showed that S. siliquosum has a greater inhibitory activity (IC 50 of 65.0 μg GAE ml −1) as compared to that of the known skin-lightening ingredient, kojic acid with an IC 50 of 109.32 μg GAE ml −1. This study is useful for improved utilization of S. siliquosum extract as a source of polyphenolic compounds important for the development of novel skin-lightening ingredient for the formulation of cosmetic products.
Full text link https://tinyurl.com/ne6z2wfx
Article title: Chemical composition and in vitro antioxidant and antibacterial activities of sargassum vulgare C. agardh from Lobo, Batangas Philippines
Authors: Eldrin De Los Reyes Arguelles, Rosario G. Monsalud, Arsenia B. Sapin
Publication title: Journal of the International Society for Southeast Asian Agricultural Sciences 25(1):112-122, June 2019
Abstract:
Seaweeds are notable in producing diverse kinds of bioactive compounds with promising pharmacological properties. A study was conducted at the National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Baños from July to November 2018 to evaluate the proximate composition and potential antioxidant and antibacterial properties of a brown macroalga, Sargassum vulgare C. Agardh. Determination of the total phenolic compounds using Folin-Ciocalteu reagent showed that the dried algal biomass have a total phenolic content of 10.13 ± 0.166 mg GAE/g. Relative antioxidant efficiency showed that S. vulgare exerted potent radical scavenging activity in a concentration dependent manner with EC50 value of 37.2 ± 0.015 μg GAE. The tested algal extract exhibited radical scavenging activity that is dose-dependent and positively correlated to its phenolic content. On the other hand, proximate composition of the dried macroalga showed that S. vulgare contains high carbohydrate, ash and crude fiber content of 34.18±0.32%, 27.09±0.00%, and 22.59±0.21 respectively. Methanolic extract of the macroalgal strain was subjected to microtiter plate dilution assay against a wide spectrum of pathogenic bacteria. S. vulgare showed pronounced activity against Staphylococcus aureus having MIC of 250 μg/ml. Aeromonas hydrophila, Bacillus cereus and Methicillin-resistant S. aureus were also moderately inhibited each having MIC of 500 μg/mL, 500 μg/mL, and 1000 μg/mL, respectively. Minimum bactericidal activity against S. aureus is higher than that of Bacillus cereus and Aeromonas hydrophila, having 500 μg/mL and 1000 μg/mL, respectively. On the other hand, Methicillin-resistant S. aureus exhibited MBC value of >1000μg/ml. This study showed the potential antioxidant and antibacterial activity of S. vulgare, which make it a suitable candidate for production of new bioactive compounds important for pharmacological and food industries.
Full text available upon request to the author